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Roberto FERNANDEZ-LAFUENTE
Roberto FERNANDEZ-LAFUENTE
Profesor de Investigacion del ICP-CSIC
Dirección de correo verificada de icp.csic.es - Página principal
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Improvement of enzyme activity, stability and selectivity via immobilization techniques
C Mateo, JM Palomo, G Fernandez-Lorente, JM Guisan, ...
Enzyme and microbial technology 40 (6), 1451-1463, 2007
38022007
Modifying enzyme activity and selectivity by immobilization
RC Rodrigues, C Ortiz, Á Berenguer-Murcia, R Torres, ...
Chemical society reviews 42 (15), 6290-6307, 2013
17842013
Potential of different enzyme immobilization strategies to improve enzyme performance
C Garcia‐Galan, Á Berenguer‐Murcia, R Fernandez‐Lafuente, ...
Advanced Synthesis & Catalysis 353 (16), 2885-2904, 2011
16852011
Glutaraldehyde in bio-catalysts design: A useful crosslinker and a versatile tool in enzyme immobilization
O Barbosa, C Ortiz, Á Berenguer-Murcia, R Torres, RC Rodrigues, ...
Rsc Advances 4 (4), 1583-1600, 2014
8232014
Control of protein immobilization: Coupling immobilization and site-directed mutagenesis to improve biocatalyst or biosensor performance
K Hernandez, R Fernandez-Lafuente
Enzyme and microbial technology 48 (2), 107-122, 2011
6812011
Stabilization of multimeric enzymes: Strategies to prevent subunit dissociation
R Fernandez-Lafuente
Enzyme and microbial technology 45 (6-7), 405-418, 2009
6722009
Strategies for the one-step immobilization–purification of enzymes as industrial biocatalysts
O Barbosa, C Ortiz, Á Berenguer-Murcia, R Torres, RC Rodrigues, ...
Biotechnology advances 33 (5), 435-456, 2015
6482015
Lipase from Thermomyces lanuginosus: uses and prospects as an industrial biocatalyst
R Fernandez-Lafuente
Journal of Molecular Catalysis B: Enzymatic 62 (3-4), 197-212, 2010
6442010
A single step purification, immobilization, and hyperactivation of lipases via interfacial adsorption on strongly hydrophobic supports
A Bastida, P Sabuquillo, P Armisen, R Fernández‐Lafuente, J Huguet, ...
Biotechnology and bioengineering 58 (5), 486-493, 1998
6421998
Immobilization of lipases by selective adsorption on hydrophobic supports
R Fernandez-Lafuente, P Armisén, P Sabuquillo, G Fernández-Lorente, ...
Chemistry and physics of lipids 93 (1-2), 185-197, 1998
5941998
Importance of the support properties for immobilization or purification of enzymes
JCS Santos, O Barbosa, C Ortiz, A Berenguer‐Murcia, RC Rodrigues, ...
ChemCatChem 7 (16), 2413-2432, 2015
5422015
Heterofunctional supports in enzyme immobilization: from traditional immobilization protocols to opportunities in tuning enzyme properties
O Barbosa, R Torres, C Ortiz, Á Berenguer-Murcia, RC Rodrigues, ...
Biomacromolecules 14 (8), 2433-2462, 2013
5082013
Novozym 435: the “perfect” lipase immobilized biocatalyst?
C Ortiz, ML Ferreira, O Barbosa, JCS dos Santos, RC Rodrigues, ...
Catalysis Science & Technology 9 (10), 2380-2420, 2019
497*2019
Immobilization of lipases on hydrophobic supports involves the open form of the enzyme
EA Manoel, JCS Dos Santos, DMG Freire, N Rueda, ...
Enzyme and Microbial Technology 71, 53-57, 2015
4912015
Different mechanisms of protein immobilization on glutaraldehyde activated supports: effect of support activation and immobilization conditions
L Betancor, F López-Gallego, A Hidalgo, N Alonso-Morales, GDOC Mateo, ...
Enzyme and microbial technology 39 (4), 877-882, 2006
4902006
Interfacial adsorption of lipases on very hydrophobic support (octadecyl–Sepabeads): immobilization, hyperactivation and stabilization of the open form of lipases
JM Palomo, G Muñoz, G Fernández-Lorente, C Mateo, ...
Journal of Molecular Catalysis B: Enzymatic 19, 279-286, 2002
4832002
Immobilization of lipases on hydrophobic supports: immobilization mechanism, advantages, problems, and solutions
RC Rodrigues, JJ Virgen-Ortíz, JCS Dos Santos, Á Berenguer-Murcia, ...
Biotechnology advances 37 (5), 746-770, 2019
4632019
Glyoxyl agarose: a fully inert and hydrophilic support for immobilization and high stabilization of proteins
C Mateo, JM Palomo, M Fuentes, L Betancor, V Grazu, F López-Gallego, ...
Enzyme and Microbial Technology 39 (2), 274-280, 2006
4472006
Increase in conformational stability of enzymes immobilized on epoxy-activated supports by favoring additional multipoint covalent attachment☆
C Mateo, O Abian, R Fernandez–Lafuente, JM Guisan
Enzyme and Microbial Technology 26 (7), 509-515, 2000
4222000
Epoxy sepabeads: a novel epoxy support for stabilization of industrial enzymes via very intense multipoint covalent attachment
C Mateo, O Abian, G Fernández‐Lorente, J Pedroche, ...
Biotechnology progress 18 (3), 629-634, 2002
3992002
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