| Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: a review O Cos, R Ramón, JL Montesinos, F Valero Microbial cell factories 5 (1), 17, 2006 | 326 | 2006 |
| Optimization of the high-level production of Rhizopus oryzae lipase in Pichia pastoris S Minning, A Serrano, P Ferrer, C Solá, RD Schmid, F Valero Journal of Biotechnology 86 (1), 59-70, 2001 | 207 | 2001 |
| Combined effect of the methanol utilization (Mut) phenotype and gene dosage on recombinant protein production in Pichia pastoris fed-batch cultures O Cos, A Serrano, JL Montesinos, P Ferrer, JM Cregg, F Valero Journal of biotechnology 116 (4), 321-335, 2005 | 127 | 2005 |
| Expression of a Rhizopus oryzae lipase in Pichia pastoris under control of the nitrogen source-regulated formaldehyde dehydrogenase promoter D Resina, A Serrano, F Valero, P Ferrer Journal of biotechnology 109 (1-2), 103-113, 2004 | 111 | 2004 |
| Sorbitol co-feeding reduces metabolic burden caused by the overexpression of a Rhizopus oryzae lipase in Pichia pastoris R Ramón, P Ferrer, F Valero Journal of Biotechnology 130 (1), 39-46, 2007 | 97 | 2007 |
| Physiological control on the expression and secretion of Candida rugosa lipase M Lotti, S Monticelli, JL Montesinos, S Brocca, F Valero, J Lafuente Chemistry and physics of lipids 93 (1-2), 143-148, 1998 | 83 | 1998 |
| Reaction scheme of lipase production byCandida rugosa growing on olive oil JL Del Rio, P Serra, F Valero, M Poch, C Sola Biotechnology letters 12 (11), 835-838, 1990 | 83 | 1990 |
| Reactivity of Pure Candida rugosa Lipase Isoenzymes (Lip1, Lip2, and Lip3) in Aqueous and Organic Media. Influence of the Isoenzymatic Profile on the Lipase … N López, MA Pernas, LM Pastrana, A Sánchez, F Valero, ML Rúa Biotechnology Progress 20 (1), 65-73, 2004 | 82 | 2004 |
| Effects of methanol on lipases: molecular, kinetic and process issues in the production of biodiesel M Lotti, J Pleiss, F Valero, P Ferrer Biotechnology journal 10 (1), 22-30, 2015 | 75 | 2015 |
| The potential use of lipases in the production of fatty acid derivatives for the food and nutraceutical industries S Ferreira-Dias, G Sandoval, F Plou, F Valero Electronic Journal of Biotechnology 16 (3), 12-12, 2013 | 75 | 2013 |
| Heterologous production of Rhizopus oryzae lipase in Pichia pastoris using the alcohol oxidase and formaldehyde dehydrogenase promoters in batch and fed-batch cultures O Cos, D Resina, P Ferrer, JL Montesinos, F Valero Biochemical Engineering Journal 26 (2-3), 86-94, 2005 | 75 | 2005 |
| Effects of different fatty acids in lipase production by Candida rugosa N Obradors, JL Montesinos, F Valero, FJ Lafuente, C Sola Biotechnology letters 15 (4), 357-360, 1993 | 75 | 1993 |
| Production of native and recombinant lipases by Candida rugosa P Ferrer, JL Montesinos, F Valero, C Solà Applied biochemistry and biotechnology 95 (3), 221-255, 2001 | 73 | 2001 |
| Developing high cell density fed‐batch cultivation strategies for heterologous protein production in Pichia pastoris using the nitrogen source‐regulated FLD1 Promoter D Resina, O Cos, P Ferrer, F Valero Biotechnology and bioengineering 91 (6), 760-767, 2005 | 71 | 2005 |
| Optimization of the heterologous production of a Rhizopus oryzae lipase in Pichia pastoris system using mixed substrates on controlled fed-batch bioprocess C Arnau, R Ramon, C Casas, F Valero Enzyme and microbial technology 46 (6), 494-500, 2010 | 70 | 2010 |
| Highly enantioselective esterification of racemic ibuprofen in a packed bed reactor using immobilised Rhizomucor miehei lipase A Sánchez, F Valero, J Lafuente, C Solà Enzyme and microbial technology 27 (1-2), 157-166, 2000 | 68 | 2000 |
| Enhancement of Candida rugosa lipase production by using different control fed‐batch operational strategies MA Gordillo, A Sanz, A Sanchez, F Valero, JL Montesinos, J Lafuente, ... Biotechnology and bioengineering 60 (2), 156-168, 1998 | 66 | 1998 |
| Transcriptional response of P. pastoris in fed-batch cultivations to Rhizopus oryzae lipase production reveals UPR induction D Resina, M Bollók, NK Khatri, F Valero, P Neubauer, P Ferrer Microbial Cell Factories 6 (1), 21, 2007 | 65 | 2007 |
| Stability studies and effect of the initial oleic acid concentration on lipase production by Candida rugosa MA Gordillo, N Obradors, JL Montesinos, F Valero, J Lafuente, C Sola Applied microbiology and biotechnology 43 (1), 38-41, 1995 | 65 | 1995 |
| Recent patents on the Pichia pastoris expression system: expanding the toolbox for recombinant protein production M Bollok, D Resina, F Valero, P Ferrer Recent Patents on Biotechnology 3 (3), 192-201, 2009 | 61 | 2009 |
