Juan M. Bolivar
Juan M. Bolivar
Department of Chemical Engineering, School of chemical Sciences,Complutense University of Madrid
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Biotransformations in microstructured reactors: more than flowing with the stream?
JM Bolivar, J Wiesbauer, B Nidetzky
Trends in biotechnology 29 (7), 333-342, 2011
1472011
Advanced characterization of immobilized enzymes as heterogeneous biocatalysts
JM Bolivar, I Eisl, B Nidetzky
Catalysis Today 259, 66-80, 2016
1152016
Coating of soluble and immobilized enzymes with ionic polymers: full stabilization of the quaternary structure of multimeric enzymes
JM Bolivar, J Rocha-Martin, C Mateo, F Cava, J Berenguer, ...
Biomacromolecules 10 (4), 742-747, 2009
1122009
Improvement of the stability of alcohol dehydrogenase by covalent immobilization on glyoxyl-agarose
JM Bolivar, L Wilson, SA Ferrarotti, JM Guisan, R Fernandez-Lafuente, ...
Journal of biotechnology 125 (1), 85-94, 2006
1002006
Stabilization of a formate dehydrogenase by covalent immobilization on highly activated glyoxyl-agarose supports
JM Bolivar, L Wilson, SA Ferrarotti, R Fernandez-Lafuente, JM Guisan, ...
Biomacromolecules 7 (3), 669-673, 2006
992006
Improvement of enzyme properties with a two-step immobilizaton process on novel heterofunctional supports
C Mateo, JM Bolivar, CA Godoy, J Rocha-Martin, BC Pessela, JA Curiel, ...
Biomacromolecules 11 (11), 3112-3117, 2010
982010
Purification and stabilization of a glutamate dehygrogenase from Thermus thermophilus via oriented multisubunit plus multipoint covalent immobilization
JM Bolivar, J Rocha-Martin, C Mateo, F Cava, J Berenguer, D Vega, ...
Journal of Molecular Catalysis B: Enzymatic 58 (1-4), 158-163, 2009
702009
Evaluation of different immobilization strategies to prepare an industrial biocatalyst of formate dehydrogenase from Candida boidinii
JM Bolivar, L Wilson, SA Ferrarotti, R Fernandez-Lafuente, JM Guisan, ...
Enzyme and microbial technology 40 (4), 540-546, 2007
682007
Shine a light on immobilized enzymes: real-time sensing in solid supported biocatalysts
JM Bolivar, T Consolati, T Mayr, B Nidetzky
Trends in biotechnology 31 (3), 194-203, 2013
592013
Positively charged mini-protein zbasic2 as a highly efficient silica binding module: Opportunities for enzyme immobilization on unmodified silica supports
JM Bolivar, B Nidetzky
Langmuir 28 (26), 10040-10049, 2012
592012
Heterofunctional supports for the one-step purification, immobilization and stabilization of large multimeric enzymes: Amino-glyoxyl versus amino-epoxy supports
JM Bolivar, C Mateo, V Grazu, AV Carrascosa, BC Pessela, JM Guisan
Process Biochemistry 45 (10), 1692-1698, 2010
592010
The presence of thiolated compounds allows the immobilization of enzymes on glyoxyl agarose at mild pH values: New strategies of stabilization by multipoint covalent attachment
JM Bolivar, F López-Gallego, C Godoy, DS Rodrigues, RC Rodrigues, ...
Enzyme and Microbial Technology 45 (6-7), 477-483, 2009
582009
Immobilization–stabilization of a new recombinant glutamate dehydrogenase from Thermus thermophilus
JM Bolivar, F Cava, C Mateo, J Rocha-Martín, JM Guisán, J Berenguer, ...
Applied microbiology and biotechnology 80 (1), 49-58, 2008
542008
Stabilization of a highly active but unstable alcohol dehydrogenase from yeast using immobilization and post-immobilization techniques
JM Bolivar, J Rocha-Martín, C Mateo, JM Guisan
Process Biochemistry 47 (5), 679-686, 2012
522012
Modulation of the distribution of small proteins within porous matrixes by smart-control of the immobilization rate
JM Bolivar, A Hidalgo, L Sánchez-Ruiloba, J Berenguer, JM Guisán, ...
Journal of biotechnology 155 (4), 412-420, 2011
512011
Multiphase biotransformations in microstructured reactors: opportunities for biocatalytic process intensification and smart flow processing
JM Bolivar, B Nidetzky
Green Processing and Synthesis 2 (6), 541-559, 2013
502013
Oriented and selective enzyme immobilization on functionalized silica carrier using the cationic binding module Zbasic2: Design of a heterogeneous D‐amino acid oxidase catalyst …
JM Bolivar, B Nidetzky
Biotechnology and bioengineering 109 (6), 1490-1498, 2012
502012
Characterization and further stabilization of a new anti-prelog specific alcohol dehydrogenase from Thermus thermophilus HB27 for asymmetric reduction of carbonyl compounds
J Rocha-Martín, D Vega, JM Bolivar, A Hidalgo, J Berenguer, JM Guisán, ...
Bioresource technology 103 (1), 343-350, 2012
492012
Oriented Immobilization of Enzymes Made Fit for Applied Biocatalysis: Non‐Covalent Attachment to Anionic Supports using Zbasic2 Module
J Wiesbauer, JM Bolivar, M Mueller, M Schiller, B Nidetzky
ChemCatChem 3 (8), 1299-1303, 2011
482011
New biotechnological perspectives of a NADH oxidase variant from Thermus thermophilus HB27 as NAD+-recycling enzyme
J Rocha-Martín, D Vega, JM Bolivar, CA Godoy, A Hidalgo, J Berenguer, ...
BMC biotechnology 11 (1), 1-11, 2011
462011
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Artículos 1–20