|Increase in conformational stability of enzymes immobilized on epoxy-activated supports by favoring additional multipoint covalent attachment☆|
C Mateo, O Abian, R Fernandez–Lafuente, JM Guisan
Enzyme and Microbial Technology 26 (7), 509-515, 2000
|Multifunctional epoxy supports: a new tool to improve the covalent immobilization of proteins. The promotion of physical adsorptions of proteins on the supports before their …|
C Mateo, G Fernández-Lorente, O Abian, R Fernández-Lafuente, ...
Biomacromolecules 1 (4), 739-745, 2000
|Epoxy sepabeads: a novel epoxy support for stabilization of industrial enzymes via very intense multipoint covalent attachment|
C Mateo, O Abian, G Fernández‐Lorente, J Pedroche, ...
Biotechnology Progress 18 (3), 629-634, 2002
|Some special features of glyoxyl supports to immobilize proteins|
C Mateo, O Abian, M Bernedo, E Cuenca, M Fuentes, ...
Enzyme and Microbial Technology 37 (4), 456-462, 2005
|Reversible enzyme immobilization via a very strong and nondistorting ionic adsorption on support–polyethylenimine composites|
C Mateo, O Abian, R Fernandez‐Lafuente, JM Guisan
Biotechnology and Bioengineering 68 (1), 98-105, 2000
|Epoxy-amino groups: a new tool for improved immobilization of proteins by the epoxy method|
C Mateo, R Torres, G Fernández-Lorente, C Ortiz, M Fuentes, A Hidalgo, ...
Biomacromolecules 4 (3), 772-777, 2003
|Co-aggregation of penicillin G acylase and polyionic polymers: an easy methodology to prepare enzyme biocatalysts stable in organic media|
L Wilson, A Illanes, O Abián, BCC Pessela, R Fernández-Lafuente, ...
Biomacromolecules 5 (3), 852-857, 2004
|Encapsulation of crosslinked penicillin G acylase aggregates in lentikats: evaluation of a novel biocatalyst in organic media|
L Wilson, A Illanes, BCC Pessela, O Abian, R Fernández‐Lafuente, ...
Biotechnology and bioengineering 86 (5), 558-562, 2004
|Stabilization of enzymes by multipoint immobilization of thiolated proteins on new epoxy‐thiol supports|
V Grazú, O Abian, C Mateo, F Batista‐Viera, R Fernández‐Lafuente, ...
Biotechnology and bioengineering 90 (5), 597-605, 2005
|Novel bifunctional epoxy/thiol-reactive support to immobilize thiol containing proteins by the epoxy chemistry|
V Grazú, O Abian, C Mateo, F Batista-Viera, R Fernández-Lafuente, ...
Biomacromolecules 4 (6), 1495-1501, 2003
|Stabilization of penicillin G acylase from Escherichia coli: site-directed mutagenesis of the protein surface to increase multipoint covalent attachment|
O Abian, V Grazú, J Hermoso, R González, JL García, ...
Appl. Environ. Microbiol. 70 (2), 1249-1251, 2004
|Reversible and strong immobilization of proteins by ionic exchange on supports coated with sulfate‐dextran|
M Fuentes, BCC Pessela, JV Maquiese, C Ortiz, RL Segura, JM Palomo, ...
Biotechnology progress 20 (4), 1134-1139, 2004
|Determination of protein‐protein interactions through aldehyde‐dextran intermolecular cross‐linking|
M Fuentes, RL Segura, O Abian, L Betancor, A Hidalgo, C Mateo, ...
Proteomics 4 (9), 2602-2607, 2004
|Preparation of artificial hyper-hydrophilic micro-environments (polymeric salts) surrounding enzyme molecules: New enzyme derivatives to be used in any reaction medium|
O Abian, L Wilson, C Mateo, G Fernández-Lorente, JM Palomo, ...
Journal of Molecular Catalysis B: Enzymatic 19, 295-303, 2002
|Stabilization of immobilized enzymes against water-soluble organic cosolvents and generation of hyper-hydrophilic micro-environments surrounding enzyme molecules|
O Abian, C Mateo, G Fernández-Lorente, JM Palomo, ...
Biocatalysis and Biotransformation 19 (5-6), 489-503, 2001
|Promotion of multipoint covalent immobilization through different regions of genetically modified penicillin G acylase from E. coli|
V Grazú, F López-Gallego, T Montes, O Abian, R González, JA Hermoso, ...
Process Biochemistry 45 (3), 390-398, 2010
|Therapeutic strategies for Gaucher disease: miglustat (NB-DNJ) as a pharmacological chaperone for glucocerebrosidase and the different thermostability of velaglucerase alfa and …|
O Abian, P Alfonso, A Velazquez-Campoy, P Giraldo, M Pocovi, J Sancho
Molecular pharmaceutics 8 (6), 2390-2397, 2011
|New cationic exchanger support for reversible immobilization of proteins|
M Fuentes, JV Maquiese, BCC Pessela, O Abian, R Fernández‐Lafuente, ...
Biotechnology progress 20 (1), 284-288, 2004
|A unified framework based on the binding polynomial for characterizing biological systems by isothermal titration calorimetry|
S Vega, O Abian, A Velazquez-Campoy
Methods 76, 99-115, 2015
|Dendrimers as potential inhibitors of the dimerization of the capsid protein of HIV-1|
R Doménech, O Abian, R Bocanegra, J Correa, A Sousa-Herves, ...
Biomacromolecules 11 (8), 2069-2078, 2010